Rupa Shree* & Nayni Saxena
University Department of Zoology, Ranchi University, Ranchi, Jharkhand, India.
Received : 24th June, 2023 ; Revised : 25th July, 2023
DOI:-https://doi.org/10.5281/zenodo.12795735
Abstract– The synthetic estrogen ethnyl estradiol (EE2) is widely used in oral contraceptives. It is an endocrine disrupting contaminant of aquatic environments. The present study examines the effects of long- term exposure of EE2 on liver tissue of male Clarias batrachus. Fishes weighing 100- 150 g (length :14 – 18 cm) were used and grouped into four different aquaria. Experimental groups were supplemented with increasing levels of EE2 as 5μg, 10 μg and 20 μg per litre of EE2 through Premarin (oral contraceptive pill). Control group fishes were not exposed to EE2. After treatment for 90 consecutive days the histological alterations in the liver were studied by Transmission Electron Microscopy. Electron microscopic studies revealed disruptive changes in the hepatocytes as evident by enlarged nuclei, indistinct nucleoli, swollen and extended mitochondria and indistinct cell membranes after EE2 exposure. Cell necrosis was clearly seen in EE2 exposed fishes only. The present results show liver damage in male Clarias batrachus after EE2 administration.
Key words: Fish, Liver, hepatocyte, Transmission Electron Microscopy
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