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Effect of phytohormones (2,4-D & KN) in callus induction of Lawsonia inermis L.

Nazra Paiker & Kunul Kandir*
University Department of Botany, Ranchi University, Ranchi, Jharkhand, India
Received : 18th December, 2021 ; Revised : 19th January, 2022

Abstract-Different concentration of phytohormones affected callus formation of Lawsonia inermis L. explant was cultured in Ms medium supplemented with different concentration of 2, 4-D and KN. Cultures were kept on 25±2ºC temperature and 16 hr photoperiod while callus was observed on different concentration of auxin and cytokinien in combination. Most suitable medium of callus formation from shoot tips was that 2, 4-D (2.5mg/L) + KN (0.5mg/L).

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