Drought stress leads to alteration of protein spots in rice, Oryza sativa L

Aparna Jha^a & Yashodhara Verma^b
aJacob School of Biotechnology & Bioengineering, SHIATS, (Formerly AAIDU), Allahabad, U.P

Received : 11th January, 2015; Revised : 9th February, 2016

Abstract : Apart from the regular circadian and seasonal perturbations, there may be certain other rapid and unpredicted disturbances in the environment resulting in stressful conditions. For instance, paucity of water for long periods due to lack of irrigation, infrequent rains or lowering of water table results in drought stress. Plants can initiate a number of molecular, cellular, and physiological changes to respond and adapt to drought stress, thus enabling them to survive. Synthesis of stress proteins is a ubiquitous response to cope with prevailing stressful conditions. We constructed here a proteome map to investigate the effect of drought stress on the rice (Oryza sativa L.) proteome. Plants were stressed for 24, 48, 72 and 96 hours. Total proteins were extracted and separated by two dimensional gel electrophoresis followed by Coomassie staining.