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Qualitative analysis of Phytohormones in brassinosteroid treated tomato plants during nematode pathogenesis

Ravinderjit Kaura, Puja Ohria* & Renu Bhardwajb
a*Dept. of Zoology, Guru Nanak Dev University, Amritsar-143005 (Punjab), India
bDept. of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, India
Received , 15th December, 2013 ;Revised: 05th February, 2014

Abstract : Phytohormones are the signalling molecules that are produced in extremely low concentrations in plants. They regulate physiological and cellular processes both locally and at far. Under stress, in addition to the physico-chemical modulations, slight alterations in the indigenous levels of these hormones, thereby, activate plant’s defence responses. Therefore, in the present study, qualitative analysis of phytohormones was carried out in brassinosteroid treated tomato plants during nematode infection. Qualitative analysis of phytohormones was carried out in whole plant material. Surface sterilized seeds of tomato cultivars Pusa Ruby (susceptible) and PNR-7 (resistant) were treated with different concentrations of 28-Homobrassinolide. Comparisons were made between Control and 10-9 M HBl in Pusa Ruby. While in PNR-7, comparisons were made between Control and 10-7 M HBl treated plants. Analysis was carried out 120hrs and 72hrs postnematode inoculation in Pusa Ruby and PNR-7 respectively. Resultsascertained the presence of three hormones viz. Putrescine, Salicylic acid and Jasmonic acid in both the treatments in cultivars. Thus, the study indicated the role of these hormones in plants under stress.

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