Manisha Ratan Horoa* & Ashok Kumar Choudharya
a*Department of Botany, Ranchi University, Ranchi
Received ,15th July, 2012 ;Revised: 25th August, 2012
Abstract : Different concentration of phytohormone affected for induction of shoot in Cardiospernum halicacabum. The nodal and leaf segment were cultured on Murashige and Skoog medium supplemented with (2, 4-D), 2, 4-Dichlorophenoxy acetic acid for callus induction. The callus were transferred to MS medium supplemented with various concentrations of (BAP) Benzylamino purnia, IAA and KN for shoot regeneration in combination.
Key words : Shoot induction , shoot regeneration, BAP,KN,2,4-D, NAA.