MSET Biospectra

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Reading: Effect of plant growth regulators on shoot regeneration on in vitro using shoot bud culture of Pongamia pinnata (Karanj)

Effect of plant growth regulators on shoot regeneration on in vitro using shoot bud culture of Pongamia pinnata (Karanj)

mset-bospectra By mset-bospectra
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Vishhwaraj Lala* & Anju Priya Akhouryb
aDept. of Biotechnology, Jamshedpur Womens College, Jamshedpur
bDept. OF Botany and Co-ordinator of Biotechnology, Jamshedpur Womens College, Jamshedpur
Received 3rd June, 2010; Revised 13th August, 2010

Abstract : Axillary shoot multiplication from shoot bud segments collected from the superior about  15 year old Pongamia pinnata(Karanj) tree were tried . Pongamia pinnata axillary bud explants receiving 2.0mg/l BA gave the height response as far as individual cytokinins are concerned . In such cultures shoots varying from 3 to 5 numbers were produced at an average of 3.0±1.62 per plant. Shoots formed in vitro were rooted on full-strength MS medium supplemented with different concentration of growth regulator.

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