Glycogen deposition in the proctodeal wall of fourth instar larvae of gram beetle, Callosobruchus chinensis Linn. infesting moong seed

Pushpalata Hansdak^a*, Kumari Tulika^b & M. Prasada
^aDepartment of Zoology, Ranchi University, Ranchi, Jharkhand, India
^bDept. of Zoology, J. P. University, Chapra, Bihar
Received 11th November, 2009; Revised 27 February, 2010

Abstract : The qualitative histogenesis of growing larval gut in stored grain pests is a function of differential deposition and incorporation of essential biomolecules in its cellular architecture. Since salivary glands in all the insects are situated along the oesophagus which is an important part of proctodeum, the quantitative deposition of glycogen in the proctodeal wall plays an important part for the larval development and metamorphosis.
In the present investigation serial histological sections of proctodeal wall of  fourth larval instar of gram beetle, Callosobruchus chinensis feeding on any moong seed were observedstained through PAS histochemical staining techniques. At five days intervals of feeding excecive deposition of glycogen has been observed with increasing in quantity with the enhanced degree of continued feeding.
The deposition of glycogen in the proctodeal wall may be a significant histochemical transformation in situ compared to the zero day fed larvae for the purpose of metabolic energy production subscribing the rapid larval growth and development.

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