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In vitro determination of allelopathic efficacy of some species of weeds on wheat (Triticum aestivum L.) seeds.

Dipti Kumari & B.K.Dayal
University Department of Botany, B.N.M.University, Madhepura, Bihar, India

Received : 03rd June, 2023 ; Revised : 02nd July, 2023
DOI:-https://doi.org/10.5281/zenodo.12194747

AbstractWeeds are unwanted problem in agriculture. In the present study allelopathic efficacy of four wild species such as Alternanthera sessilis, Calotropis procera, Parthenium hysterophorus and Chenopodium album were used to study their allelopathic impact on wheat. Aqueous extracts of the above weeds were prepared and their impact on percentage germination of seeds, growth of plumule, radical, total dry biomass were observed with respect to percentage germination of seeds at 5% concentration. Aqueous extract of Parthenium hysterophorus was more effective where only 18% seeds could germinate. This was followed by aqueous extract of Calotropis procera 20%, Alternanthera sessilis 22% and less effect was observed in case of Chenopodium album that was 36%. Similarly, the reduction in length of plumule was the highest in the seedlings treated with 5% aqueous extract of leaves of Calotropis procera that was 2.60cm, followed by Parthenium hysterophorus 2.40 cm, Alternanthera sessilis 2.7cm and 3.10 cm in case of Chenopodium album. This was also with root length which was 2.20cm at 5% concentration of the aqueous extract of Calotropis procera, followed by 2.30 cm in case of Parthenium hysterophorus and 2.8cm case of Chenopodium album. The dry biomass of seedlings was also lower at 5% concentration in aqueous extract of leaves of Calotropis procera (260 mg) followed by Parthenium hysterophorus (280mg), Alternanthera sessilis (282mg) and least in case of Chenopodium album (320mg) chlorophyll content of the seedling was also reduced in comparison to the control. Here also aqueous extract of Calotropis procera affected the much which was 0/mg/g of leaf. Here also Chenopodium had least impact. All the parameters revealed gradual reduction in comparison to control along with the increasing concentration of the aqueous extracts of leaves of selected weeds.

Key words: Allelopathy, Aqueous extract, Weeds, dry biomass

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