Green Synthesis of Zinc oxide nanoparticles using stem extract of Withania somnifera and its antibacterial effect

Pinki Kumari^a, M. Anwar Mallick^a* & Navin Chandra^b
aUniversity Department of Biotechnology Vinoba Bhave University, Hazaribag, Jharkhand, India
^bUniversity Department of Physics, Vinoba Bhave University, Hazaribag, Jharkhand, India

Received : 06^th June, 2023 ; Revised : 08^th July, 2023
DOI:-https://doi.org/10.5281/zenodo.12028265

Abstract– In this study, we report the synthesis of Zinc oxide nanoparticles highly explored in the field of nanotechnology, biotechnology, medicine and others fields. The nanoparticles were successfully synthesized by the acetate derivatives of zinc oxide and plant stem extract of the indigenous ayurvedic plant Withania somnifera. 10 mM of Zn (CH₃COO)₂. 2H₂O was mixed with the plant stem extract at different concentration and the reaction temperature was maintained at 65^oC. The white colour paste obtained was dried, collected and packed for characterization. In UV-visible Spectrophotometer analysis absorption peak was observed at 303 nm, which is specific for Zinc Oxide nanoparticles. The hydrodynamic diameter of the synthesized nanoparticles ZnO NPs was observed to be 232 nm with zeta potential 13 ± 11 mV. The Field Emission Scanning Electron Microscope (FESEM) characterization reveals the presence of zinc oxide nanoparticle. In its clustered form, the average size of nanoparticles was measured to be 100 nm. All nanoparticles showed different degree of antibacterial activity against Gram Positive Micrococcus luteus (ATCC4698), Staphylococcus aureus (BAA2686) and Gram-negative bacteria Escherichia coli (PTA8019) and Salmonella typhi (ATCC29630). The antibacterial activity was inversely proportional to the size of the synthesized zinc oxide nanoparticles.

Key words: Green synthesis, Characterization, Zinc oxide nanoparticles and Antibacterial activity

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