Sacchi Snehaa*, S.Mauryab & A.K Choudharya
aUniversity Department of Botany, Ranchi University, Ranchi, Jharkhand, India
bICAR-RCER, Research Centre, Palandu, Ranchi, Jharkhand, India
Received : 11th June, 2021 ; Revised : 12th July, 2021
Abstract– Knowing the antifungal efficacies of large numbers of botanical extracts in short span of time were carried out by Thin Layer Chromatography bioassay against selected fungi. Methanol, Ethanol and Ethyl acetate extracts of botanical spices namely, Garlic (Allium sativum), Nutmeg (Myristica fragrans), Black pepper (Piper nigrum), Ajwain (Trachyspermum ammi), Chebulic myrobalan (Terminalia chebula), Fenugreek (Trigonella foenumgraecum) and Ginger (Zingiber officinale) were isolated. The obtained botanical extracts were subjected through TLC bioassay and tested against selected phytopathogens namely Alternaria capsici (Capsicum) and Curvularia lunata (Oryzae sativa). The separated compound were subjected to bioassay by spraying spore suspension (104cfu/ml) of selected fungi and incubated for 24-36 hrs at 25±2oC. After incubation; zone of inhibition were recorded and on that basis antifungal potential of the extracts were determined. All the organic fractions of Garlic showed antifungal properties while methanol extract of nutmeg, ethanol extracts of Ajwain and Chebulic myrobalan showed highest zone of inhibition against both the test fungi. The presence of these active principles with definite Rf value in spices extracts when prepared as bioformulations or established in crop rotation could be exploited as an organic inputs towards eco-friendly, alternatives of chemical fungicides in disease management strategies.
Key words: Thin layer chromatography, Botanical, A. capsici, C. lunata, Inhibition.