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Antimicrobial activity of formulated herbal extract against Dental Plaque isolates

Sushila Kumaria, Sugandha Singhb & Birendra Prasada
aMicrobial and Molecular Genetics Laboratory, Department of Botany, Patna University, Bihar, India
bNational Institute of Pathology, ICMR, Safdarjung Hospital, Delhi, India
Received : 24th April, 2020 ; Revised : 25th May, 2020

Abstract- An increase in the drug resistance attributes of bacteria as well as negative changes in oral mucosa in regards to the extensive use of chemical based broad spectrum antiseptics against dental caries has resulted in rise of the demand of potential, safe and inexpensive herbal medicines. Therefore the present study was conducted to evaluate and compare the antimicrobial activity of fruit extract of three plants Amalaki (Phyllanthus emblica) Bibhitaki (Terminalia bellirica) and Haritaki (Terminalia chebula) either alone or in combined formulation (Triphala) against cryogenic pathogen combined with dental plaque. To check antimicrobial property, Zone of Inhibition and Minimum Inhibitory Concentration (MIC) of these extracts were calculated. The herbal formulation reduces > 55% in vitro bacterial colony forming units (CFU) causing dental plaque over a period of 2 weeks. Median Lethal Dose (L50) of herbal formulation was found to be 500µg/ml. After reckoning all the antimicrobial activities, Triphala formulation was found better than the individual extract and also comparable to most commonly used antiplaque agent Chlorhexidine. Therefore to reduce the risks associated with the use of synthetic antiplaque agents Triphala formulation can be used as a potent substitute for antiseptics.

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