Influence of percent seed germination of three cabbage cultivars in different inoculum concentration of the bacterium Xanthomonas campestris pv. campestris

Anil Kumar^a*
^a*Department of Botany, Ranchi College, Ranchi, Jharkhand, India.

Received , 15th October  2013 :Revised: 70th December; 2013

Abstract :The effectiveness of inoculum concentration for seed germination in three different cabbage cultivars has been studied. Seeds were obtained from Kanke near Birsa Agriculture University, Ranchi. Black rot of crucifer caused by the bacterium Xanthomonas campestris pv. campestris(Xcc).This disease induces most effective symptoms in cabbage cultivars. The inoculum strength of 10¹, 10², 10⁴ and 10⁶ CFU/ml of Xanthomonas campestris pv. campestris were taken. Seeds were pin pricked and dipped in each of the four inoculum concentration separately for 5, 15 and 30 minutes for 1, 2, 3, 6,9 and 12 days. The 5 min of inoculation dip was insufficient for affecting seed mortality. 15 and 30 min of inoculation dip had almost similar effect on seed mortality. The best inoculum concentration for affecting seed mortality was found 10⁶ CFU/ml in the three cabbage cultivars Green Express, Krishna and Pusa drumhead respectively. Apparent seed mortality was observed for initial few days followed by overall delayed seed germination.